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MJ Research ptc-100 programmable thermal controller
Ptc 100 Programmable Thermal Controller, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptc-100+programmable+thermal+controller/ptc+100+thermocycler/pmc11884422-46-20-24
Average 90 stars, based on 1 article reviews
ptc-100 programmable thermal controller - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens
Article Snippet: The concentration of total RNA in each sample was measured using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA). .. RNA samples were reverse transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PTC-100 programmable thermal controller (MJ Research, Waltham, MA), programmed according to the manufacturer's instructions. .. Real-time PCR was performed using an AriaMX real-time PCR system (Agilent Technologies, Santa Clara, CA) with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies). lists the primers used for the PCR.

Article Title: Interleukin 1 Receptor Antagonist (IL‐1ra) Production and Expression Dynamics in Mammary Glands After Lipopolysaccharide Infusion
Article Snippet: The concentration of each sample was assessed using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA was reverse‐transcribed using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co. Ltd. Osaka, Japan) and PTC‐100 programmable thermal controller (MJ Research, Waltham, MA, USA), following the manufacturer's instructions. .. Real‐time PCR was performed using the AriaMX RT‐PCR system (Agilent Technologies, Santa Clara, CA, USA) with the Brilliant III Ultra‐Fast SYBR Green QPCR Master Mix (Agilent Technologies).

Article Title: Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens.
Article Snippet: The concentration of total RNA in each sample was measured using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA). .. RNA samples were reverse transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PTC-100 programmable thermal controller (MJ Research, Waltham, MA), programmed according to the manufacturer’s instructions. .. Real-time PCR was performed using an AriaMX real-time PCR system (Agilent Technologies, Santa Clara, CA) with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies).

Real-time Polymerase Chain Reaction:

Article Title: Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens
Article Snippet: The concentration of total RNA in each sample was measured using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA). .. RNA samples were reverse transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PTC-100 programmable thermal controller (MJ Research, Waltham, MA), programmed according to the manufacturer's instructions. .. Real-time PCR was performed using an AriaMX real-time PCR system (Agilent Technologies, Santa Clara, CA) with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies). lists the primers used for the PCR.

Article Title: Interleukin 1 Receptor Antagonist (IL‐1ra) Production and Expression Dynamics in Mammary Glands After Lipopolysaccharide Infusion
Article Snippet: The concentration of each sample was assessed using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA was reverse‐transcribed using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co. Ltd. Osaka, Japan) and PTC‐100 programmable thermal controller (MJ Research, Waltham, MA, USA), following the manufacturer's instructions. .. Real‐time PCR was performed using the AriaMX RT‐PCR system (Agilent Technologies, Santa Clara, CA, USA) with the Brilliant III Ultra‐Fast SYBR Green QPCR Master Mix (Agilent Technologies).

Article Title: Intravaginal injection of Lactobacillus johnsonii may modulates oviductal microbiota and mucosal barrier function of laying hens.
Article Snippet: The concentration of total RNA in each sample was measured using NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA). .. RNA samples were reverse transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PTC-100 programmable thermal controller (MJ Research, Waltham, MA), programmed according to the manufacturer’s instructions. .. Real-time PCR was performed using an AriaMX real-time PCR system (Agilent Technologies, Santa Clara, CA) with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies).

Polymerase Chain Reaction:

Article Title: Assessing the molecular genetics of attention networks
Article Snippet: Taq polymerase, PCR buffer and dNTPs were obtained from QIAGEN and used at recommended concentrations for a 20 ul PCR reaction. .. PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications. ..

Article Title: Disentangling Vector-Borne Transmission Networks: A Universal DNA Barcoding Method to Identify Vertebrate Hosts from Arthropod Bloodmeals
Article Snippet: .. PCR reactions were carried out using a PTC-100 Programmable Thermal Controller (MJ Research) in a final volume of 30 μl containing 1 unit of a commercial Taq Polymerase (Bioline), 1X manufacturer-supplied buffer (Bioline), 2.5 mM MgCl 2 , 0.25 mM of each dNTP, 5% DMSO, 10 μg of BSA (Bovine Serum Albumin - Amersham corp.), 0.16 μM of primers M13BCV-FW and BCV-RV1 and 1 μl of extracted DNA. ..

Marker:

Article Title: Assessing the molecular genetics of attention networks
Article Snippet: Taq polymerase, PCR buffer and dNTPs were obtained from QIAGEN and used at recommended concentrations for a 20 ul PCR reaction. .. PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications. ..

Amplification:

Article Title: Generation of Doubled Haploid Transgenic Wheat Lines by Microspore Transformation
Article Snippet: .. Amplification was performed in a PTC-100 programmable thermal controller (MJ Research, Cambridge, MA) at 97°C for 1 min, annealing at 58°C for 20 sec and extension at 72°C for 30 sec followed by 29 cycles of 97°C for 30 sec, 60°C for 20 sec and 72°C for 1 min; this was followed by 5 min at 72°C. ..

Article Title: NS1 Specific CD8 + T-Cells with Effector Function and TRBV11 Dominance in a Patient with Parvovirus B19 Associated Inflammatory Cardiomyopathy
Article Snippet: .. T-PreAmp of this primer/gene assay pool was carried for 14 cycles on a PTC-100 Programmable Thermal Controller (MJ Research, Watertown, Massachusetts, USA) with the following program: denaturation at 95°C for 10 min and 14 cycles of amplification (15 sec at 95°C, 4 min at 60°C). ..

other:

Article Title: Protocol for extracting and isolating porcine bone-marrow-derived macrophages from ribs
Article Snippet: PTC-100 programmable thermal controller , MJ Research , N/A.



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